cardiovascularКардиоваскулярная терапия и профилактикаCardiovascular Therapy and Prevention1728-88002619-0125«SILICEA-POLIGRAF» LLCcardiovascular-1730Research ArticleЭКСПЕРИМЕНТАЛЬНАЯ КАРДИОЛОГИЯЭКСПЕРИМЕНТАЛЬНАЯ КАРДИОЛОГИЯВлияние триметазидина на энергетическую активность митохондрийTrimetazidine effects on mitochondria energetic activityЕлисееваС. В.EliseevaS. V.astei@rinet.ruГрачевС. В.GrachevS. V.astei@rinet.ruЕгороваН. Д.EgorovaN. D.astei@rinet.ruГлезерМ. Г.GlezerM. G.astei@rinet.ruАсташкинЕ. И.AstashkinE. I.astei@rinet.ruМосковская медицинская академия им. И.М.Сеченова. МоскваI.M. Sechenov Moscow Medical Academy. Moscow200820122008786469Copyright © Елисеева С.В., Грачев С.В., Егорова Н.Д., Глезер М.Г., Асташкин Е.И., 20082008Елисеева С.В., Грачев С.В., Егорова Н.Д., Глезер М.Г., Асташкин Е.И.Eliseeva S.V., Grachev S.V., Egorova N.D., Glezer M.G., Astashkin E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cardiovascular.elpub.ru/jour/article/view/1730Цель

Цель. Изучить каким образом триметазидин (TMZ) оказывает непосредственное влияние на окислительное фосфорилирование интактных митохондрий (МХ), изолированных из животных, не подвергавшихся процессу ишемии-реперфузии.

Материал и методы

Материал и методы. Объектом исследования служили МХ печени крысы, выделяемые общепринятым методом. Окислительную и фосфорилирующую функции МХ изучали полярографически. TMZ использовали в концентрации 10-4М и 10-5М.

Результаты

Результаты. Под влиянием TMZ в концентрации 10-4М и 10-5М снизилось время фосфорилирования добавленного аденозиндифосфата (АДФ), уменьшилось количество кислорода (О2), для его фосфорилирования, в результате чего увеличился коэффициент АДФ/О. Такие изменения свидетельствуют о том, что увеличилась скорость процесса фосфорилирования и его эффективность, т. е. расходование О2 стало более экономным, а образование аденозинтрифосфата возросло. При воздействии TMZ увеличился дыхательный контроль, что также свидетельствует о повышении сопряженности процессов окисления и фосфорилирования. Показано, что сам по себе TMZ не вызывает разобщения этих процессов.

Заключение

Заключение. Улучшение энергетических функций МХ является одним из ключевых факторов в цитопротекторном действии TMZ.

Aim

Aim. To investigate how trimetazidine (TMZ) directly affects oxidative phosphorylation in intact mitochondria (MCH), isolated from the animals not undergoing ischemia-reperfusion process.

Material and methods

Material and methods. Rat liver MCH were isolated by standard methods. Oxidative and phosphorylating MCH functions were studied by polarigraphic methods. TMZ was used in the concentrations of 10-4 М and 10-5 М.

Results

Results. TMZ in concentrations of 10-4 М and 10-5 М decreased the time of added adenosine diphosphate (ADPH) phosphorylation, reduced the amount of oxygen (O2) necessary for this phosphorylation, and increased ADPH/O2 coefficient. Therefore, phosphorylation velocity and effectiveness increased, i.e. O2 use became more economical, and ATPH synthesis increased. TMZ also increased breath control, which pointed to oxidation and phosphorylation association. TMZ per se did not separate these two processes.

Conclusion

Conclusion. MCH energetic function improvement is one of the key factors in TMZ cytoprotective effects.

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The authors declare that there are no conflicts of interest present.