cardiovascularКардиоваскулярная терапия и профилактикаCardiovascular Therapy and Prevention1728-88002619-0125«SILICEA-POLIGRAF» LLC10.15829/1728-8800-2020-2730cardiovascular-2730Research ArticleБИОБАНКИРОВАНИЕBIOBANKINGСбор и хранение ДНК-содержащего биоматериала и выделенной ДНКCollection and storage of DNA-containing biomaterial and isolated DNAhttps://orcid.org/0000-0002-0554-9911ДолудинЮ. В.DoludinYu. V.

Юрий Валерьевич Долудин — научный сотрудник лаборатории “Банк биологического материала”

Москва

 

stryider@rambler.ruhttps://orcid.org/0000-0003-1500-3696ЛимоноваАлена СергеевнаLimonovaA. S.

Алена Сергеевна Лимонова. — лаборант лаборатории клиномики

Москва

ALimonova@gnicpm.ruhttps://orcid.org/0000-0002-3843-6980КозловаВ. А.KozlovaV. A.

Виктория Александровна Козлова — лаборант-исследователь лаборатории “Банк биологического материала” pt'> материала”

Москва

vkozlova2905@gmail.comhttps://orcid.org/0000-0002-3081-8415ЕфимоваИ. А.EfimovaA. I.

Ирина Александровна Ефимова — лаборант-исследователь лаборатории “Банк биологического материала”

Москва

irishka90910@yandex.ruhttps://orcid.org/0000-0003-4020-6647БорисоваА. Л.BorisovaA. L.

Анна Львовна Борисова. — ведущий инженер лаборатории “Банк биологического материала”

Москва

aborisova@gnicpm.ruhttps://orcid.org/0000-0001-5989-6233МешковА. Н.MeshkovA. N.

Алексей Николаевич Мешков — Кандидат медицинских наук, руководитель лаборатории молекулярной генетики 

Москва

meshkov@lipidclinic.ruhttps://orcid.org/0000-0001-6985-7131ПокровскаяМ. С.PokrovskayaM. S.mpokrovskaia@list.ruhttps://orcid.org/0000-0002-4453-8430ДрапкинаО. МDrapkinaO. M.

Оксана Михайловна Драпкина — доктор медицинских наук, профессор, член-корр. РАН, директор

Москва

drapkina@gnicpm.ruФГБУ "Национальный медицинский исследовательский центр терапии и профилактической медицины" Минздрава РоссииРоссияNational Medical Research Center for Therapy and Preventive MedicineRussian Federation20202412202019627302730Copyright © Долудин Ю.В., Лимонова А.С., Козлова В.А., Ефимова И.А., Борисова А.Л., Мешков А.Н., Покровская М.С., Драпкина О.М., 20202020Долудин Ю.В., Лимонова А.С., Козлова В.А., Ефимова И.А., Борисова А.Л., Мешков А.Н., Покровская М.С., Драпкина О.М.Doludin Y.V., Limonova A.S., Kozlova V.A., Efimova A.I., Borisova A.L., Meshkov A.N., Pokrovskaya M.S., Drapkina O.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cardiovascular.elpub.ru/jour/article/view/2730

Развитие биомедицины сопровождается появлением новых технологий, методов диагностики и лечения, а также возможностей применения в медицинской практике новых типов биологических мишеней, в частности, нуклеиновых кислот. В качестве объектов генетических исследований используют геномную дезоксирибонуклеиновую кислоту (ДНК), внеклеточную ДНК (вкДНК) и ДНК микробиоты, получаемые из разных типов образцов: тканей, крови и ее производных, кала и др. Использование новых технологий для ДНК-анализа потребовало разработки стандартизованных методов процессинга биообразцов с целью получения качественных образцов ДНК. В научно-медицинских исследованиях применяются различные методы сбора, пробоподготовки и хранения разных видов ДНК-содержащего биоматериала, выделенной ДНК и методы оценки качества биообразцов, а также стандарты биобанкирования. Очевидно, что использование единых стандартов позволит проводить крупномасштабные генетические исследования на базе биобанков и отдельных научных лабораторий. Активное участие во внедрении единых стандартов биобанкирования принимают специалисты профессиональных организаций, таких как ISBER (International Society for Biological and Environmental Repositories), BBMRI-ERIC (Biobanking and BioMolecular Resources Research Infrastructure-European Research Infrastructure Consortium), ESBB (European, Middle Eastern & African Society for Biopreservationa and Biobanking) и российская Национальная ассоциация биобанков и специалистов по биобанкированию (НАСБИО).

The advances of biomedicine include the new technologies, diagnosis and treatment techniques, as well as the practical use of new types of biological targets, in particular, nucleic acids. Genomic deoxyribonucleic acid (DNA), extracellular DNA (exDNA) and microbiome DNA obtained from different types of samples (tissues, blood and its derivatives, feces, etc.) are used as objects of genetic research. The use of new technologies for DNA analysis required the development of standardized methods for processing biological samples in order to obtain high-quality DNA samples. The research uses various methods for collecting, preparing samples and storing various DNA-containing biomaterials and isolated DNA, as well as methods for assessing the quality of samples and biobank standards. It is obvious that the use of uniform standards will allow large-scale genetic research on the basis of biobanks and research laboratories. Specialists from professional organizations such as International Society for Biological and Environmental Repositories (ISBER), Biobanking and BioMolecular Resources Research Infrastructure-European Research Infrastructure Consortium (BBMRI-ERIC), European, Middle Eastern & African Society for Biopreservationa and Biobanking (ESBB) and the Russian National Association of Biobanks and Biobanking Professionals.

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The authors declare that there are no conflicts of interest present.