cardiovascularКардиоваскулярная терапия и профилактикаCardiovascular Therapy and Prevention1728-88002619-0125«SILICEA-POLIGRAF» LLC10.15829/1728-8800-2023-3691cardiovascular-3691Research ArticleБИОБАНКИРОВАНИЕBIOBANKINGКриоконсервация гемопоэтических стволовых клеток периферической крови в трансплантологии: современное состояние и перспективыCryostorage of peripheral blood hematopoietic stem cells in transplantology: current status and prospectshttps://orcid.org/0000-0003-3061-6108КитО. И.KitO. I.

Олег Иванович Кит — доктор медицинских наук, профессор, академик РАН, генеральный директор.

Ростов-на-Дону

Rostov­on­ Don

onko-sekretar@mail.ruhttps://orcid.org/0000-0002-3691-3317ГненнаяН. В.GnennayaN. V.

Надежда Владимировна Гненная — младший научный сотрудник лаборатории клеточных технологий.

Ростов-на-Дону

Rostov­on­ Don

ngnennaya@inbox.ruhttps://orcid.org/0000-0002-4558-5896ФилипповаС. Ю.FilippovaS. Yu.

Светлана Юрьевна Филиппова — научный сотрудник лаборатории клеточных технологий.

Ростов-на-Дону

Rostov­on­ Don

filsv@yandex.ruhttps://orcid.org/0000-0002-4555-8556ЧембароваТ. В.ChembarovaT. V.

Татьяна Владимировна Чембарова — младший научный сотрудник лаборатории клеточных технологий.

Ростов-на-Дону

Rostov­on­ Don

tanyshamova@mail.ruhttps://orcid.org/0000-0003-4457-3815ЛысенкоИ. Б.LysenkoI. B.

Ирина Борисовна Лысенко — доктор медицинских наук, профессор, заведующий отделением онкогематологии.

Ростов-на-Дону

Rostov­on­ Don

iralyss@rambler.ruhttps://orcid.org/0000-0002-6496-9641НовиковаИ. А.NovikovaI. A.

Инна Арнольдовна Новикова — доктор медицинских наук, зам. генерального директора по науке.

Ростов-на-Дону

Rostov­on­ Don

novikovainna@yahoo.comhttps://orcid.org/0000-0001-7032-8595РозенкоЛ. Я.RozenkoL. Ya.

Людмила Яковлевна Розенко — доктор медицинских наук, профессор, врач-радиотерапевт отделения радиотерапии № 2.

Ростов-на-Дону

Rostov­on­ Don

rozenkolya@rnioi.ruhttps://orcid.org/0000-0002-2565-1518ДимитриадиС. Н.DimitriadiS. N.

Сергей Николаевич Димитриади — доктор медицинских наук, врач-уролог отделения онкоурологии.

Ростов-на-Дону

Rostov­on­ Don

dimitriadi@yandex.ruhttps://orcid.org/0000-0001-7742-4918ШалашнаяЕ. В.ShalashnayaE. V.

Елена Владимировна Шалашная — кандидат биологических наук, старший научный сотрудник лаборатории изучения патогенеза злокачественных опухолей.

Ростов-на-Дону

Rostov­on­ Don

eshalashnaya@mail.ruhttps://orcid.org/0000-0002-5300-1213ИшонинаО. Г.IshoninaO. G.

Оксана Георгиевна Ишонина — кандидат биологических наук, заведующий отделом подготовки и переподготовки специалистов.

Ростов-на-Дону

Rostov­on­ Don

patentrnioi@mail.ruФГБУ "Национальный медицинский исследовательский центр онкологии" Минздрава РоссииNational Medical Research Center of Oncology2023101220232211Биобанкирование36913691Copyright © Кит О.И., Гненная Н.В., Филиппова С.Ю., Чембарова Т.В., Лысенко И.Б., Новикова И.А., Розенко Л.Я., Димитриади С.Н., Шалашная Е.В., Ишонина О.Г., 20232023Кит О.И., Гненная Н.В., Филиппова С.Ю., Чембарова Т.В., Лысенко И.Б., Новикова И.А., Розенко Л.Я., Димитриади С.Н., Шалашная Е.В., Ишонина О.Г.Kit O.I., Gnennaya N.V., Filippova S.Y., Chembarova T.V., Lysenko I.B., Novikova I.A., Rozenko L.Y., Dimitriadi S.N., Shalashnaya E.V., Ishonina O.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cardiovascular.elpub.ru/jour/article/view/3691

Трансплантация гемопоэтических стволовых клеток (ГСК) периферической крови хорошо зарекомендовала себя как процедура лечения гематологических, онкологических и аутоиммунных заболеваний. У онкологических больных трансплантация ГСК позволяет использовать в процессе лечения высокодозные цитотоксические препараты в сочетании с лучевой терапией, что обеспечивает выраженный противоопухолевый эффект. Гематологическая токсичность подобного лечения устраняется последовательным введением стволовых клеток, способствующих восстановлению ростка кроветворения. Перед трансплантацией ГСК периферической крови подвергают процедуре сбора и криоконсервации с целью дальнейшего хранения. Важным требованием, предъявляемым к криоконсервации, является сохранение жизнеспособности ГСК, ответственных за восстановление ростка кроветворения. Цель обзора — анализ литературы, посвященной изучению влияния различных способов криоконсервации ГСК периферической крови человека на сохранение жизнеспособности клеток после размораживания, а также развитие нежелательных явлений у пациентов. Рассмотрены вопросы, связанные с использованием различных криопротекторов, а также методы хранения трансплантатов ГСК. Представленные данные свидетельствуют о необходимости дальнейшего изучения влияния криопротекторов на организм человека и клеточный состав трансплантата и усовершенствования протоколов криоконсервации ГСК.

Peripheral blood hematopoietic stem cell (HSC) transplantation is a well-established procedure for the treatment of hematological, cancer and autoimmune diseases. In cancer patients, HSC transplantation allows the use of high-dose cytotoxic drugs in combination with radiation therapy during treatment, which provides a pronounced antitumor effect. The hematological toxicity of such treatment is eliminated by the sequential introduction of stem cells, which contribute to hematopoiesis restoration. Before transplantation, peripheral blood HSCs are subjected to collection and cryopreservation for further storage. An important requirement for cryopreservation is viable HSCs responsible for hematopoietic restoration. The aim of the review was to analyze the literature devoted to the influence of various methods of cryopreservation of human peripheral blood HSCs on the preservation of cell viability after thawing, as well as the development of adverse events in patients. Issues related to the use of various cryoprotectants, as well as methods for storing HSC grafts, are considered. The presented data indicate the need for further study of the effect of cryoprotectants on the human body and the cellular composition of the graft and improvement of protocols for HSC cryopreservation.

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The authors declare that there are no conflicts of interest present.