cardiovascularКардиоваскулярная терапия и профилактикаCardiovascular Therapy and Prevention1728-88002619-0125«SILICEA-POLIGRAF» LLC10.15829/1728-8800-2024-4179SQPPKZcardiovascular-4179Research ArticleОБЗОРЫ ЛИТЕРАТУРЫREVIEW ARTICLESФакторы преаналитического этапа, влияющие на уровни циркулирующих микроРНК плазмы и сыворотки кровиPreanalytical factors affecting the plasma and serum levels of circulating microRNAshttps://orcid.org/0000-0002-8395-4146СотниковаЕ. А.SotnikovaE. A.

Евгения Андреевна Сотникова — с.н.с. лаборатории молекулярной генетики Института персонализированной терапии и профилактики.

Москва

Moscow

sotnikova.evgeniya@gmail.comhttps://orcid.org/0000-0003-4765-8021КиселеваА. В.KiselevaA. V.

Анна Витальевна Киселева — к.б.н., в.н.с., руководитель лаборатории молекулярной генетики Института персонализированной терапии и профилактики.

Москва

Moscow

sanyutabe@gmail.comhttps://orcid.org/0000-0001-5989-6233МешковА. Н.MeshkovA. N.

Алексей Николаевич Мешков — д.м.н., руководитель Института персонализированной терапии и профилактики.

Москва

Moscow

meshkov@lipidclinic.ruФГБУ "Национальный медицинский исследовательский центр терапии и профилактической медицины" Минздрава РоссииNational Medical Research Center for Therapy and Preventive Medicine2024301220242311Биобанкирование41794179Copyright © Сотникова Е.А., Киселева А.В., Мешков А.Н., 20252025Сотникова Е.А., Киселева А.В., Мешков А.Н.Sotnikova E.A., Kiseleva A.V., Meshkov A.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cardiovascular.elpub.ru/jour/article/view/4179

Циркулирующие микрорибонуклеиновые кислоты (микроРНК) являются перспективными биомаркерами различных заболеваний, однако их использование в клинических лабораторных условиях требует высокочувствительных, воспроизводимых, надежных и устойчивых методов, позволяющих проводить их точную количественную оценку в плазме и сыворотке крови. Преаналитическая фаза исследований, проводимых с использованием биообразцов, состоит из их сбора, обработки, хранения и транспортировки. Преаналитические условия остаются основными искажающими факторами в исследованиях микроРНК, а стандартизация этих условий, осуществляемая в биобанках, может оказать положительное влияние на воспроизводимость результатов исследований и возможность их сравнения. Целью обзора является рассмотрение основных современных оригинальных исследований, посвященных изучению преаналитических факторов, которые являются важным источником различий в исследованиях, посвященных циркулирующим микроРНК, на этапах от взятия крови до получения плазмы или сыворотки.

Circulating microribonucleic acids (microRNAs) are promising biomarkers of various diseases, but their clinical laboratory use requires highly sensitive, reproducible, reliable and sustainable methods for their accurate plasma and serum quantification. The preanalytical phase of studies conducted using biospecimens consists of their collection, processing, storage and transportation. Preanalytical conditions remain the main distorting factors in microRNA studies, and standardization of these conditions, carried out in biobanks, can improve the reproducibility of results and their comparison. The review aim is to consider the main contemporary original studies on preanalytical factors, which are an important source of variability in studies on circulating microRNAs at the stages from blood collection to plasma or serum production.

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The authors declare that there are no conflicts of interest present.