cardiovascularКардиоваскулярная терапия и профилактикаCardiovascular Therapy and Prevention1728-88002619-0125«SILICEA-POLIGRAF» LLC10.15829/1728-8800-2014-3-11-17cardiovascular-50Research ArticleЭПИДЕМИОЛОГИЯ И ПРОФИЛАКТИКАEPIDEMIOLOGY AND PREVENTIONВЗАИМОСВЯЗЬ ФАКТОРОВ СЕРДЕЧНО-СОСУДИСТОГО РИСКА И БИОЛОГИИ ТЕЛОМЕР С ПРИЗНАКАМИ СОСУДИСТОГО СТАРЕНИЯINTERRELATION BETWEEN CARDIOVASCULAR RISK FACTORS AND TELOMERE BIOLOGY WITH THE SIGNS OF VASCULAR AGINGКругликоваА. С.KruglikovaA. S.

м. н.с. отдела комплексного снижения риска неинфекционных заболеваний

anna.delta@mail.ruСтражескоИ. Д.StrajeskoI. D.

к. м.н., в. н.с. отдела

anna.delta@mail.ruТкачеваО. Н.TkachevaO. N.

д. м.н., профессор, руководитель отдела

anna.delta@mail.ruАкашеваД. У.AkashevaD. U.

к. м.н., в. н.с. отдела

anna.delta@mail.ruПлоховаМ. В.PlokhovaE. V.

аспирант отдела

anna.delta@mail.ruПыхтинаВ. С.PykhtinaV. S.

лаборант отдела

anna.delta@mail.ruДудинскаяЕ. В.DudinskayaE. V.

к. м.н., в. н.с. отдела

anna.delta@mail.ruИсайкинаО. Ю.IsaykinaO. Yu.

к. м.н., с. н.с. лаборатория применения амбулаторных диагностических методов в профилактике хронических неинфекционных заболеваний

anna.delta@mail.ruШарашкинаН. В.SharashkinaN. V.

к. м.н., м. н.с. отдела комплексного снижения риска неинфекционных заболеваний

anna.delta@mail.ruОзероваИ. Н.OzerovaI. N.

к. б.н, в. н.с. отдела изучения биохимических маркеров риска хронических неинфекционных заболеваний

anna.delta@mail.ruВыгодинВ. А.VygodinV. A.

с. н.с.

anna.delta@mail.ruГомырановаН. В.GomyranovaN. V.

рук. клинико-диагностической лаборатории

anna.delta@mail.ruФГБУ “Государственный научно-исследовательский центр профилактической медицины” Минздрава России, МоскваFSBH State Research Centre for Preventive Medicine of the Ministry of Health. Moscow, Russia2014200620141331117Copyright © Кругликова А.С., Стражеско И.Д., Ткачева О.Н., Акашева Д.У., Плохова М.В., Пыхтина В.С., Дудинская Е.В., Исайкина О.Ю., Шарашкина Н.В., Озерова И.Н., Выгодин В.А., Гомыранова Н.В., 20142014Кругликова А.С., Стражеско И.Д., Ткачева О.Н., Акашева Д.У., Плохова М.В., Пыхтина В.С., Дудинская Е.В., Исайкина О.Ю., Шарашкина Н.В., Озерова И.Н., Выгодин В.А., Гомыранова Н.В.Kruglikova A.S., Strajesko I.D., Tkacheva O.N., Akasheva D.U., Plokhova E.V., Pykhtina V.S., Dudinskaya E.V., Isaykina O.Y., Sharashkina N.V., Ozerova I.N., Vygodin V.A., Gomyranova N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://cardiovascular.elpub.ru/jour/article/view/50Цель

Цель. Изучить взаимосвязи факторов сердечно-сосудистого риска, процессов клеточного и сосудистого старения.

Материал и методы

Материал и методы. В исследование были включены 136 человек без клинических проявлений сердечно-сосудистых заболеваний, сахарного диабета 2 типа, медикаментозной терапии, но с одним или несколькими факторами сердечно-сосудистого риска (курение, артериальная гипертония, ожирение, дислипидемия, гипергликемии натощак). Длина теломер и активность теломеразы были измерены с помощью полимеразной цепной реакции. Толщина комплекса интима-медиа (ТКИМ) и наличие атеросклеротических бляшек (АСБ) измерены с помощью дуплексного сканирования правых и левых сонных артерий. Скорость распространения пульсовой волны (СРПВ) была измерена методом аппланационной тонометрии. Биохимические анализы выполнены стандартными лабораторными методами.

Результаты

Результаты. СРПВ достоверно коррелировала с возрастом, индексом массы тела, уровнем гликированного гемоглобина, гликемией натощак и длиной теломер. Наличие у пациентов АСБ и увеличенной ТКИМ достоверно коррелировало с возрастом, индексом массы тела, артериальной гипертонией, нарушениями липидного обмена.

Заключение

Заключение. Увеличенная артериальная жесткость и проявления сублинического атеросклероза имеют разные причины. Уровень СРПВ в большей степени связан с нарушениями углеводного обмена, в то время как ТКИМ и наличие АСБ связаны с липидными нарушениями.

Aim

Aim. To study interrelationship between cardiovascular risk factors and cellular and vascular aging processes. Material and methods. Totally 136 patients were included having no signs of cardiovascular diseases, diabetes 2nd type and receiving no drug therapy, but with one or several risk factors for cardiovascular diseases (smoking, arterial hypertension, obesity, dyslipidemia, fasting hyperglycemia). The telomere length and telomerase activity was measured by polymerase chain reaction. The thickness of intima-media complex (TIMC) and presence of atherosclerotic plaques (ASP) were measured by duplex scanning of right and left carotid arteries. Pulse wave velocity (PWV) was measured by applanation tonometry. Biochemical tests done by standard.

Results

Results. PWV significantly correlated with age, body mass index, glycosilated hemoglobin level, fasting glycemia and telomere length. Presence of ASP and increased TIMC significantly correlated with age, body mass index, arterial hypertension, dyslipidemia. Conclusion. Increase of arterial wall stiffness and subclinical atherosclerotic disease have different causes. The level of PWV more linked with carbohydrate metabolism disorder, and TIMC and ASP are linked with lipid disorders.

старение cocyдовжесткость сосудовтеломе- рыфакторы сердечно-сосудистого рискауглеводный обменлипидный обменvascular agingarterial stiffnesstelomerescardiovascular risk factorscarbohydrate metabolismlipid metabolismReferencesWHO Global InfoBase (http: www.infobase.who.int).WHO Global InfoBase (http: www.infobase.who.int).Naghavi M, Falk E, Hecht HS, et al. From vulnerable plaque to vulnerable patient-part III: executive summary of the screening for heart attack prevention and education (SHAPE) task force report. Am J Cardiology 2006; 98 (2): 2H-15.Naghavi M, Falk E, Hecht HS, et al. From vulnerable plaque to vulnerable patient-part III: executive summary of the screening for heart attack prevention and education (SHAPE) task force report. Am J Cardiology 2006; 98 (2): 2H-15.Strazhesko I D, Akasheva DU, Dudinskaya E N, Tkacheva ON. Aging vessels: main features and mechanisms. Cardiovascular Therapy and Prevention 2012; 11 (4): 93–100. Russian (Стражеско И. Д., Акашева Д. У., Дудинская Е. Н., Ткачева О. Н. Старение сосудов: основные признаки и механизмы. Кардиоваскулярная терапия и профилактика 2012; 11 (4): 93–100).Strazhesko I D, Akasheva DU, Dudinskaya E N, Tkacheva ON. Aging vessels: main features and mechanisms. Cardiovascular Therapy and Prevention 2012; 11 (4): 93–100. Russian (Стражеско И. Д., Акашева Д. У., Дудинская Е. Н., Ткачева О. Н. Старение сосудов: основные признаки и механизмы. Кардиоваскулярная терапия и профилактика 2012; 11 (4): 93–100).Madeira IR, Carvalho CNM, Mussi F, et al. Cut-off point for Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index established from Receiver Operating Characteristic (ROC) curve in the detection of metabolic syndrome in overweight prepubertal children. Arq Bras EndocrinolMetab 2008; 52: 9 Sгo Paulo Dec.Madeira IR, Carvalho CNM, Mussi F, et al. Cut-off point for Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index established from Receiver Operating Characteristic (ROC) curve in the detection of metabolic syndrome in overweight prepubertal children. Arq Bras EndocrinolMetab 2008; 52: 9 Sгo Paulo Dec.Cawthon RM. Telomere measurement by quantitative PCR. Nucleic Acids Res 2002; 30 (10): e47.Cawthon RM. Telomere measurement by quantitative PCR. Nucleic Acids Res 2002; 30 (10): e47.Kim NW. Specific association of human telomerase activity with immortal cells and cancer. Science 1994; 266 (5193): 2011–5.Kim NW. Specific association of human telomerase activity with immortal cells and cancer. Science 1994; 266 (5193): 2011–5.Boutouyrie P. New techniques for assessing arterial stiffness. Diabetes&Metabolism 2008; 34: 21–6.Boutouyrie P. New techniques for assessing arterial stiffness. Diabetes&Metabolism 2008; 34: 21–6.Stein JH, Korcarz CE, Hurst RT, et al. Use of Carotid Ultrasound to Identify Subclinical Vascular Disease and Evaluate Cardiovascular Disease Risk: A Consensus Statement from the American Society of Echocardiography Carotid Intima-Media Thickness Task Force Endorsed by the Society for Vascular Medicine. J Am Soc Echocardiography 2008; 48: 93–111.Stein JH, Korcarz CE, Hurst RT, et al. Use of Carotid Ultrasound to Identify Subclinical Vascular Disease and Evaluate Cardiovascular Disease Risk: A Consensus Statement from the American Society of Echocardiography Carotid Intima-Media Thickness Task Force Endorsed by the Society for Vascular Medicine. J Am Soc Echocardiography 2008; 48: 93–111.Goh SY, Cooper ME. The role of advanced glycation end products in progression and complications of diabetes. J Clin Endocrinol Metab 2008; 93: 1143–52.Goh SY, Cooper ME. The role of advanced glycation end products in progression and complications of diabetes. J Clin Endocrinol Metab 2008; 93: 1143–52.Konova E, Baydanoff S, Atanasova M, Velkova A. Age-related changes in the glycation of human aortic elastin. Exp Gerontol 2004; 39: 249–54.Konova E, Baydanoff S, Atanasova M, Velkova A. Age-related changes in the glycation of human aortic elastin. Exp Gerontol 2004; 39: 249–54.Kilhovd BK, Juutilainen A, Lehto S, et al. High serum levels of advanced glycation end products predict increased coronary heart disease mortality in nondiabetic women but not in nondiabetic men: a population based 18 year follow up study. Arterioscler Thromb Vasc Biol 2005; 25: 815–20.Kilhovd BK, Juutilainen A, Lehto S, et al. High serum levels of advanced glycation end products predict increased coronary heart disease mortality in nondiabetic women but not in nondiabetic men: a population based 18 year follow up study. Arterioscler Thromb Vasc Biol 2005; 25: 815–20.Scuteri A, Najjar SS, Muller DC, et al. Metabolic syndrome amplifies the age-associated increases in vascular thickness and stiffness. JACC 2004; 43:1388–95.Scuteri A, Najjar SS, Muller DC, et al. Metabolic syndrome amplifies the age-associated increases in vascular thickness and stiffness. JACC 2004; 43:1388–95.Ceriello A. Postprandial Hyperglycaemia: A new Risk Factor for Cardiovascular Disease. Diabetes, Metabolism, and the Heart 2008; 17: 363–73.Ceriello A. Postprandial Hyperglycaemia: A new Risk Factor for Cardiovascular Disease. Diabetes, Metabolism, and the Heart 2008; 17: 363–73.Mackey RH, Sutton-Tyrrell K, Vaitkevicius PV, et al. Correlates of aortic stiffness in elderly individuals: a subgroup of the Cardiovascular Health Study. Am J Hypertens 2002; 15 (1 Pt 1):16–23.Mackey RH, Sutton-Tyrrell K, Vaitkevicius PV, et al. Correlates of aortic stiffness in elderly individuals: a subgroup of the Cardiovascular Health Study. Am J Hypertens 2002; 15 (1 Pt 1):16–23.Otsuka T, Kawada T, Ibuki C, et al. Obesity as an independent influential factor for reduced radial arterial wave reflection in a middle aged Japanese male population. Hypertens Res 2009; 32 (5): 387–91.Otsuka T, Kawada T, Ibuki C, et al. Obesity as an independent influential factor for reduced radial arterial wave reflection in a middle aged Japanese male population. Hypertens Res 2009; 32 (5): 387–91.Okuda K, Khan MY, Skurnick J, et al. Telomere attrition of the human abdominal aorta: relationship with age and atherosclerosis. Atherosclerosis 2000; 152: 391–8.Okuda K, Khan MY, Skurnick J, et al. Telomere attrition of the human abdominal aorta: relationship with age and atherosclerosis. Atherosclerosis 2000; 152: 391–8.Oeseburg H, Westenbrink BD, de Boer RA, et al. Can critically short telomeres cause functional exhaustion of progenitor cells in postinfarction heart failure. JACC 2007; 50:1911–2.Oeseburg H, Westenbrink BD, de Boer RA, et al. Can critically short telomeres cause functional exhaustion of progenitor cells in postinfarction heart failure. JACC 2007; 50:1911–2.De Meyer T, Rietzschel ER, De Buyzere ML, et al. Systemic telomere length and preclinical atherosclerosis: the Asklepios study. Eur. Heart J 2009; 30: 3074–81.De Meyer T, Rietzschel ER, De Buyzere ML, et al. Systemic telomere length and preclinical atherosclerosis: the Asklepios study. Eur. Heart J 2009; 30: 3074–81.Scuteri A, Najjar SS, Muller DC, et al. Metabolic syndrome amplifies the ageassociated increases in vascular thickness and stiffness. JACC 2004; 43: 1388–95.Scuteri A, Najjar SS, Muller DC, et al. Metabolic syndrome amplifies the ageassociated increases in vascular thickness and stiffness. JACC 2004; 43: 1388–95.

The authors declare that there are no conflicts of interest present.