Assessment of the reliability of serological monitoring results based on biobank materials
https://doi.org/10.15829/1728-8800-2023-3709
Abstract
Aim. To evaluate the safety of immunoglobulins (Ig) of different classes (IgA, IgM, IgG, IgE) under conditions of long-term low-temperature storage of blood serum samples.
Material and methods. The work used samples of blood serum from the collection of the Department of Epidemiology, examined by enzyme immunoassay for antibodies of classes IgA, IgM, IgG, IgE twice as follows: immediately upon receipt in the laboratory and after storage at a temperature of -70о C for 8 years.
Results. After the storage of seropositive sera, the level of IgA antibodies did not change significantly (p=0,7). For the remaining classes of immunoglobulins (IgM, IgG, IgE), a small (not >15%) but significant decrease (p<0,05), most pronounced for IgE, was observed. It was shown that the higher the antibody level in the samples during the first study, the more it decreased after long-term storage (correlation at p<0,05). This did not lead to false negative results, which is due to the greater accuracy in measuring small values.
Conclusion. Long-term low-temperature storage of serum samples in biobank conditions ensures the safety of antibodies of different classes and is the basis for the reliability of future studies.
Supporting agencies: The work was supported by the State assignment of the Ministry of Education and Science of the Russian Federation № FZWN-2020-0017 (design, analysis and interpretation of data)
About the Authors
A. V. Nozdracheva
National Center of Epidemiology and Microbiology
Russian Federation
Russian Federation
Moscow
T. A. Semenenko
National Center of Epidemiology and Microbiology
Russian Federation
Russian Federation
Moscow
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Supplementary files
What is already known about the subject?
- The use of blood serum bank materials allows for large-scale research in epidemiology of infectious diseases, including the study of population immunity. An experimental study was carried out on the safety of immunoglobulins IgA, IgM, IgG, IgE during long-term low-temperature storage of serum samples.
What might this study add?
- Storage of blood serum samples for 8 years at a temperature of -70оC had a slight effect on the level of immunoglobulins IgA, IgM, IgG, IgE. The higher the antibody level in the samples during the initial study, the more it decreased in the reevaluation, which, however, did not change the interpretation of the study results.
Review
For citations:
Nozdracheva A.V., Semenenko T.A. Assessment of the reliability of serological monitoring results based on biobank materials. Cardiovascular Therapy and Prevention. 2023;22(11):3709. (In Russ.) https://doi.org/10.15829/1728-8800-2023-3709
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