Creation of a cell culture bank: experience of the Pokrovsky Cell Technology Center
https://doi.org/10.15829/1728-8800-2024-4173
EDN: KVZRMX
Abstract
Modern research in cell biology places high demands on the model objects used, including cell cultures. These requirements are dictated by the need to avoid artifacts in work and reduce potential risks to the environment associated with biological hazard. The article presents the experience of creating cell culture bank for personal and public storage at the Pokrovsky Cell Technology Center. The main points of managing the collection, incoming quality control, certification, processing, preparation for storage and storage of human biological materials are considered. The developed approaches and work algorithms made it possible to create a collection of early passage cultures consisting of certified samples that meet the requirements for cell cultures for the manufacture of high-tech drugs and biomedical cell products, as well as for research.
About the Authors
E. M. PrikhodkoRussian Federation
Saint Petersburg
O. V. Supilnikova
Russian Federation
Saint Petersburg
I. V. Maslennikova
Russian Federation
Saint Petersburg
N. I. Yenukashvili
Russian Federation
Saint Petersburg
A. V. Kotova
Russian Federation
Saint Petersburg
V. V. Bagaeva
Russian Federation
Saint Petersburg
T. L. Zolina
Russian Federation
Saint Petersburg
A. I. Konkina
Russian Federation
Saint Petersburg
G. S. atsemirsky
Russian Federation
Saint Petersburg
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Supplementary files
What is already known about the subject?
- When creating cell culture biobanks for the production of high-tech drugs and for conducting studies, researchers encounter difficulties with insufficiently developed regulatory framework, the absence of strict criteria for culture quality control.
What might this study add?
- The developed methods, including quality assurance and control, made it possible to create the largest collection of cultures of mesenchymal stromal cells, fibroblasts, early-passaged human umbilical vein endothelial cells in the North-West region, consisting of certified samples that meet existing cell culture requirements for the production of high-tech drugs, as well as for research.
Review
For citations:
Prikhodko E.M., Supilnikova O.V., Maslennikova I.V., Yenukashvili N.I., Kotova A.V., Bagaeva V.V., Zolina T.L., Konkina A.I., atsemirsky G.S. Creation of a cell culture bank: experience of the Pokrovsky Cell Technology Center. Cardiovascular Therapy and Prevention. 2024;23(11):4173. (In Russ.) https://doi.org/10.15829/1728-8800-2024-4173. EDN: KVZRMX