Effect of plasma and serum storage conditions on circulating microRNA levels
https://doi.org/10.15829/1728-8800-2024-4180
EDN: KMPGLG
Abstract
Over the past decade, circulating small non-coding ribonucleic acid molecules (microRNAs) have demonstrated their potential as minimally invasive diagnostic and prognostic biomarkers of various diseases. Standardization of preanalytical and analytical factors, including collection, processing and storage of biosamples, plays a significant role in the reliability and reproducibility of circulating microRNA quantification. To date, there is no consensus regarding the data normalization used in the analysis of circulating microRNA expression. The review aim is to consider modern original papers on various storage conditions of biobanked plasma and serum samples with subsequent isolation of circulating microRNAs for analysis.
Keywords
microRNA,
plasma,
serum,
standardization,
storage,
extraction,
preanalytical factors,
analytical factors
About the Authors
E. A. Sotnikova
National Medical Research Center for Therapy and Preventive Medicine
Russian Federation
Russian Federation
Moscow
A. V. Kiseleva
National Medical Research Center for Therapy and Preventive Medicine
Russian Federation
Russian Federation
Moscow
A. N. Meshkov
National Medical Research Center for Therapy and Preventive Medicine
Russian Federation
Russian Federation
Moscow
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Supplementary files
What is already known about the subject?
- MicroRNAs (small non-coding ribonucleic acid molecules) are involved in post-transcriptional regulation of gene expression and affect various pathological conditions.
- Standardization of preanalytical variables is a critical step in clinical implementation as well as in research of circulating microRNAs.
What might this study add?
- Maximum stability of microRNA is achieved by storing serum and plasma at -80оC while minimizing the number of freeze-thaw cycles.
Review
For citations:
Sotnikova E.A., Kiseleva A.V., Meshkov A.N. Effect of plasma and serum storage conditions on circulating microRNA levels. Cardiovascular Therapy and Prevention. 2024;23(11):4180. (In Russ.) https://doi.org/10.15829/1728-8800-2024-4180. EDN: KMPGLG
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