POLYMORPHISM OF THE MATRIX METALLOPROTEASES 2 AND 9 AND AORTIC STIFFNESS IN ADULTS WITH ARTERIAL HYPERTENSION

Aim. To study the parameters of aortic stiffness and polymorphisms of matrix metalloproteases genes MMP2 (-1306) and MMP9 (-1564) in patients with arterial hypertension (AH) of young and middle age.

Material and methods. Totally, 58 patients studied with AH, age 25-60 y.o., and 59 healthy volunteers. The pulse wave velocity (PWV) assessed, and augmentation index (arteriography on TensioClinic TL1, Hungary). The specimens of DNA extracted, from blood leucocytes, and gene typing of promotor regions of the genes MMP2 (-1306) and MMP9 (-1564).

Results. Genotypes MMP2 (-1306) (CT and ТТ) are found in AH patients with the signs of metabolic syndrome (MS) 1,72 times more commonly than in controls (p=0,23). Genotypes MMP9 (-1564) (CT and ТТ) are slightly more common in the general AH group comparing to controls (OR=1,4; p=0,39). In AH patients with no MS signs the genotypes MMP9 (-1564) (CT and ТТ) are predominant and found 2,66 times more frequently, than in the healthy (р=0,04). In cases of high PWV in AH, genotype MMP9 (-1564) (СТ and ТТ) is found 1,9 times more often than in controls with AH and normal PWV with no statistical significance (р=0,14).

Conclusion. The polymorphism MMP9 (-1564 C/T) finding in AH patients can be used for prediction of additional risk for cardiovascular catastrophes with the individualized monitoring of collected factors. 

1. Mancia G, Fagard R, Narkiewicz K, et al. The 2013 ESH/ESC Guidelines for the Management of Arterial Hypertension. European heart journal 2013; 2159-219.

2. Diagnosis and treatment of hypertension. Clinical guidelines. Moscow; 2013 Russian (Диагностика и лечение артериальной гипертонии. Клинические рекомендации. Москва; 2013).

3. Ryden L, Grant PJ, Anker SD et al. ESC Guidelines on diabetes, prediabetes, and cardiovascular diseases developed in collaboration with the EASD. Diabetes and Vascular Disease Research 2014; 11 (3): 133-73.

4. Panchenko EA, Nevzorova VA, Belov PS, et al. Polymorphism of matrix metalloproteinases 2 and 9 genes in patients with myocardial infarction combained with metabolic syndrome. Fundamental research 2014; 10: 1964-70. Russian (Панченко Е.А., Невзорова В.А., Белов П.С. и др. Полиморфизм генов матриксных металлопротеиназ 2 и 9 у пациентов при инфаркте миокарда и метаболическом синдроме. Фундаментальные исследования 2014; 10: 1964-70).

5. Tamura K, Dudley J, Nei M. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 2011; 28(10): 2731-9.

6. Nevzorova V, Brodskaia T, Sakovskaia A, et al. Condition of aortic stiffness and adipokines in the blood serum of young and middle-aged patients with essential hypertension. Pacific Medical J 2015; 1: 43-7. Russian (Невзорова В.А., Бродская Т.А., Саковская А.В. и др. Состояние жесткости аорты и содержание адипокинов в сыворотке крови у пациентов молодого и среднего возраста с гипертонической болезнью. Тихоокеанский медицинский журнал 2015; 1: 43-7).

7. Rogoza AN, Kaveshnikov VS, Trubacheva IA, et al. The state of the vascular wall in adult population of Tomsk in the framework of the project ESSAY-RF. System Hypertension 2014; 4: 42-9. Russian (Рогоза А.Н., Кавешников В.С., Трубачева И.А. и др. Состояние сосудистой стенки в популяции взрослого населения на примере жителей города Томска, по данным исследования ЭССЕ-РФ. Системные гипертензии 2014; 4: 42-9).

8. Ben-Shlomo Y, Spears M, Boustred C, et al. Aortic pulse wave velocity improves cardiovascular event prediction: an individual participant meta-analysis of prospective observational data from 17,635 subjects. JACC 2014; 63(7): 636-46.

9. Zhou S, Feely J, Spiers J, et al. Matrix metalloproteinase-9 polymorphism contributes to blood pressure and arterial stiffness in essential hypertension. J Hum Hypertens 2007; 21: 861-7.

10. Li J, Lu H, Tao F, et al. Meta-analysis of MMP9-562C/T and the risk of coronary heart disease. Cardiology 2013; 124(1): 53-9.

11. Lin RT, Chen CH, Tsai PC, et al. Sex-specific effect of matrix Metalloproteinase-9 functional promoter polymorphism on carotid artery stiffness. J Atherosclerosis 2012; 416-20.

12. Huang R, Deng L, Shen AN, et al. Associations of MMP1, 3, 9 and TIMP3 Genes Polymorphism with Isolated Systolic Hypertension in Chinese Han Population. International J Med Scie 2013; 10(7): 840-7.

13. Yadav SS, Mandal RK, Singh MK, et al. High serum level of matrix metalloproteinase 9 and promoter polymorphism — 1562 C:T as a new risk factor for metabolic syndrome. DNA Cell Biol 2014; 33(11): 816-22.

14. Lacchini R, Jacob-Ferreira AL, Luizon MR, et al. Common matrix metalloproteinase 2 gene haplotypes may modulate left ventricular remodelling in hypertensive patients. J Human Hypertension 2012; 26: 171-7.