Creation of a biosample collection from patients after myocardial infarction in the Khanty Mansi Autonomous Okrug — Yugra
A V Morozkina,
A S Vorobev,
M Yu Donnikov,
L V Kovalenko,
M L Safronova,
A Kh Gapurova,
K Yu Nikolaev https://doi.org/10.15829/1728-8800-2023-3805
Abstract
Aim. Creation of a sample collection of whole blood, leukocyte suspension, genomic deoxyribonucleic acid from patients with myocardial infarction for fundamental and applied research in medicine.
Materials and methods. The collection, transportation and processing of biomaterial samples in the biobank of the Medical Institute of Surgut State University were carried out according to standard methods using recommendations, standard operating procedures and national guidelines for biobanking.
Results. As of September 1, 2023, the collection has collected 744 aliquots of whole blood and leukocyte suspension and isolated 108 samples of genomic deoxyribonucleic acid from 108 donors after myocardial infarction. Aliquots of samples from the biobank collection are actively used for a number of molecular genetic studies (genotyping, massively parallel sequencing).
Conclusion. This biosample collection on the basis of Surgut State University provides current and future research projects in cardiology with high-quality biomaterial.
Supporting agencies: The study was financially supported by the Ugra Scientific and Technological Development Foundation within the research project № 2022-05-04
About the Authors
A V Morozkina
Surgut State University
Russian Federation
Russian Federation
PhD, Cand. Sci. (Bio), leadingresearcher of the scientific SurGU
A S Vorobev
Surgut State University; District Cardiac Dispensary "Center for Diagnostic and Cardiovascular Surgery"
Russian Federation
Russian Federation
PhD, Cand. Sci. (Med), Associate Professor of the Department
of Cardiology
M Yu Donnikov
Surgut State University
Russian Federation
Russian Federation
PhD, Cand. Sci. (Med), doctor-laboratory geneticist, leading
researcher of the scientific SurGU
L V Kovalenko
Surgut State University
Russian Federation
Russian Federation
Dr. Sci. (Med.), Director of the Medical Institute, Head of the
Department of Pathophysiology and General Pathology
M L Safronova
Surgut State University
Russian Federation
Russian Federation
master's student of the Institute of Natural and Technical Sciences, expert of the Center for collective use
A Kh Gapurova
Surgut State University
Russian Federation
master's student of the Institute of Natural and Technical Sciences, laboratory assistant of the Center for collective Use
Russian Federation
master's student of the Institute of Natural and Technical Sciences, laboratory assistant of the Center for collective Use
K Yu Nikolaev
Surgut State University; Research Institute of Internal and Preventive Medicine — branch of the Federal Research Center Institute of Cytology and Genetics
Russian Federation
Russian Federation
Dr. Sci. (Med.), Professor of the Department of Cardiology, Head of the Laboratory of Emergency Therapy of the Research Institute of Therapy and Prevention
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Supplementary files
What is already known about the subject?
- Thanks to the standardization of work, biobanks provide high quality preserved samples of biomaterial for a variety of types of biomedical research.
- A collection of biomaterial samples from cardiovascular patients with detailed information allows for both retrospective analysis and prediction of the outcomes of socially significant diseases.
What might this study add?
- A unique collection of biological samples from patients after myocardial infarction, unique for the Khanty-Mansi Autonomous Okrug — Yugra, was collected and described.
- The quality of samples and accompanying information allows the effective use of biomaterial in molecular genetic research, including in cooperation between different research teams.
Review
For citations:
Morozkina A.V., Vorobev A.S., Donnikov M.Yu., Kovalenko L.V., Safronova M.L., Gapurova A.Kh., Nikolaev K.Yu. Creation of a biosample collection from patients after myocardial infarction in the Khanty Mansi Autonomous Okrug — Yugra. Cardiovascular Therapy and Prevention. 2023;22(11):3805. (In Russ.) https://doi.org/10.15829/1728-8800-2023-3805
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